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eBay Amscope 40X-1000X Trinocular Inverted Epi-fluorescence Microscope w/9MP Color Global Shutter Camera Sku: IN300TC-FL-9MG3 Key Features 40X to 1000X expanded magnification range using a quadruple lens turret and additional 25X eyepieces for seven unique settings. An inverted epi-fluorescence microscope with blue and green excitation blocks and a high-power HBO arc-lamp for broad-spectrum coverage. The inverted format is a stable, ergonomic platform for cell cultures, multi-well plates, and larger specimen containers. The infinity-corrected optical system ensures high-quality, high-resolution images. Specialized 10X and 20X objective lenses are included for phase-contrast applications. The trinocular photo port provides a dedicated interface for mounting a camera. Includes a highly-sensitive 9MP USB 3.0 camera with global-shutter technology and specialized microscopy software for PC, Mac, and Linux The IN300T-FL is an inverted fluorescence microscope built for professional laboratories and universities focused on live-cell studies and a range of genetic research. It provides all of the necessary features for fluorescence, brightfield, and phase-contrast microscopy in an intuitive and economical design. Plan-fluor lenses provide a higher level of optical corrections and resolution for improved clarity. The Viewing Head The viewing head has a 45° incline and Siedentopf interpupillary adjustment for flexibility and comfort. Super-widefield optics provide a larger field-of-view so you can see more specimen at any magnification. For users with astigmatism or asymmetrical vision, the right ocular can be adjusted to fine-tune focus. The trinocular format provides a dedicated photo-port for mounting a microscopy camera for capturing photos or videos. The 23mm photo port is height-adjustable to match focus with the eyepieces. Optics The infinity-corrected optical system eliminates optical errors and ensures highly precise images. The quadruple objective turret provides instant access to 4 magnification levels. This allows you to efficiently move through magnifications from 40X to 400X. Additional 25X eyepieces expand the maximum magnification to 1000X with 7 unique levels. These high-quality lenses are semi-apochromatically corrected for superior optical corrections and transmittance, with planatic flat-field correction to provide edge-to-edge sharpness. The Stage The large stage can accommodate slides, multi-well plates, dishes, and short flasks. Two plates are supplied for the stage’s aperture, including glass and key-holed metal for varying light control. A large mechanical caliper can hold and position any of the sample templates for precision placement. Transmitted Illumination The transmitted illumination system uses a fixed-height, 30W incandescent light-source with integrated condenser, and slots for filters and a phase-annulus board. The annulus board provides two centerable phase-contrast annuli, as well as an open setting which can be used with colored filters for band-isolation or color-correction. Phase Contrast The 10X and 20X objective lenses are equipped with phase rings for phase-contrast observation. By sliding the provided phase-annulus board into the overhead lamp, the transmitted lighting is quickly changed to work with the objective lenses to enhance contrast in hard-to-see samples such as live cells which cannot be stained. By manipulating variances in phase caused by changing refractive indices, once imperceptible details can present as transitions from dark to light. Epi-fluorescence Illumination For fluorescence applications, a 100W mercury-vapor arc lamp provides a powerful, broad-spectrum light-source for excitation. The included blue and green excitation filter blocks cover a large range of popular fluorophores. Optional ultraviolet and violet excitation filters are also available. Fluorescence Filters Filter Set Excitation Filter Emission Filter B 450-490nm 520nm G 510-560nm 590nm Mercury HBO Excitation Mercury HBO lamps produce a wide spectrum of wavelengths, including ultraviolet. While this fluorescent light-source can excite fluorophores throughout its entire spectrum, there are peak wavelengths with optimal output. The included filter blocks will accommodate a wide range of fluorophores, although the nature of mercury arc lamps limits effectiveness outside of the peak wavelengths. Band Peak Wavelength UV 334nm, 365nm V 405nm B 436nm G 546nm Y 578nm Digital Imaging The highly-sensitive 9MP camera uses a cutting-edge 1″-format Sony global-shutter sensor. The camera is designed to excel in low-light with high maximum framerates, and eliminates rolling-shutter distortion. The camera captures still images and video, and allows you to view a live stream on your PC. With software available for Windows, Mac, and Linux, it’s a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features and specialized tools for fluorescence color-mapping. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features. Specifications Optical System Infinity-corrected Head Trinocular, 45° incline Interpupillary Adjustment Siedentopf compensation-free gemel, 50mm-75mm Ocular Diameter 30mm Ocular Diopters right ocular Eyepieces 10X, 25X Photo-port height-adjustable 23mm tube Objective Lenses Infinity-corrected Objective Turret Quadruple Focusing System Focus Adjustment Coaxial Coarse/Fine, adjustable tension Fine Division 0.002mm Stage fixed-height, mechanical X-Y translation Stage Dimensions 172mm x 242mm Stage X-Y Travel Range 75mm x 40mm Specimen Holders Terasaki plate, Petri dish Episcopic Illumination (Fluorescence) 100W mercury HBO arc-lamp, centerable Transmitted Illumination 12V 30W halogen variable intensity Condenser NA 0.3, iris diaphragm, filter slot Power 110VAC 60Hz Objective Lenses Magnification Corrections NA Working Distance Cover-glass Thickness 4X plan fluor 0.15 28mm 1.5mm 10X plan fluor, phase-contrast 0.30 3mm 1.5mm 20X plan fluor, phase-contrast 0.50 4.27mm 1.5mm 40X plan fluor 0.60 2.23mm 1.5mm Camera Specifications Sensor Type CMOS (color) Sensor Optical Format 1″ Active Pixels 9M (4096 x 2160) Pixel Size 3.45µm x 3.45µm Active Sensor Area 14.13mm x 7.45mm Shutter global shutter Sensitivity 1146mV @ 1/30s Spectral Response 380-650nm with IR-cut filter Capture Resolution and Maximum Framerate 34fps @ 4096×2160 60fps @ 2048×1080 Connectivity USB 3.0 Lens-mount C-mount Compensating Lens 1X Power 5VDC over USB Software OS Requirements Windows (32/64 bit) XP/Vista/7/8/10, Mac OS 10.8+, Linux kernel 3.13+ Hardware Requirements Intel Core2 2.8GHz or comparable processor, 4GB RAM Features Windows Version Live video preview Captures still images and video Time-lapse capture Exposure and white-balance controls Dark-field correction Digital binning Image processing Measurement tools, including lines, arcs, polygons Segmentation and counting tools Image-stitching, extended depth-of-field, HDR Fluorescence color-mapping Mac/Linux Versions Live video preview Captures still images and video Exposure control Color adjustment Measurement tools, including lines, arcs, polygons Packing List: One microscope body Four objectives lenses: 4X, 10X(Ph), 20X(Ph) and 40X One pair 10X eyepieces One pair 25X eyepieces One phase-contrast annulus board One top-mount illuminator One rear-mount fluorescence illuminator One power supply for fluorescence illuminator One UV blocking shield One 30W halogen bulb One 100W mercury bulb One dust cover Color filters Two power cords One C-mount USB camera One USB 3.0 Type B to USB 3.0 type A cable One software CD Shipping & Handling: FREE Shipping to Contiguous USA (the lower 48 States) Return Policy We offer 30 days from the date of receipt (verified by tracking information) to request a return on a unit. To do so, please send us a message through eBay member to member messages, with your reason for return, requesting an RMA number. Note that shipping costs are borne by the customer. Returns requested after 30 days are not valid, and any returns sent without an RMA number will be returned to sender at the sender’s expense. Also, please insure all items shipped back, as damage to items by the courier during the return shipment to us are not covered otherwise. Please keep all original packing materials, as they are required for return (or a 10% restocking fee will be charged). Returned items must be in new/usable condition unless defective on arrival to you. Warranty Information We, AmScope, provide all warranty services. Please feel free to contact us whenever you need help by messaging us from the store you purchased the product from. Five Year Warranty for Microscopes – All our microscopes come with a five (5) year factory warranty against manufacturing defects, covering labor and parts. One Year Warranty for Accessories – All our microscope accessories (USB cameras, illuminators, illumination, dust covers, and etc.) come with a one (1) year warranty. Note: Buyers bear the cost of shipping and handling cost round trip for warranty services. Warranty does not cover consumable accessories and parts such as bulbs. Warranty service times vary throughout the year, and are processed in a first come, first serve manner, when parts are available and in stock. Most repairs/replacements are done within 1-2 weeks, however if there is a long line during the busy season, or if your parts need to be ordered from our manufacturer, processing times can be up to 6-8 weeks. We appreciate your patience and understand everyone has a critical need for their unit, and do everything we can to ensure that these requests are handled as quickly as possible. Defective/Missing Items For defective items or any missing items from your order, please contact us right away to let us know of the issue. We allow a 30 day period (from the date of receipt of the item in the mail) to report such issues–any items missing after that time frame will need to be purchased, and any defective items will be treated as warranty issues for repair or replacement only (no returns for refund). For missing items and broken items, we do require a picture of the shipping label on the box, and of the items that arrived with your unit for verification before we can send any missing items or replacements to you. Return Procedure To initiate an RMA, please send us a message through the store you purchased it from. Include your order number, reason for return, and if any pictures are required for your claim, please include them also. From there, we will process within 24-72 hours, and if approved, issue you an RMA number and instructions on where to mail your unit back to. If qualifying for return shipping covered by us (for defective items within 30 days of receipt), your return label may take another 24-48 hours to process and be received in your email inbox. Follow the issued instructions, and once received, we will process the item in, and check to ensure everything is there. We will contact you when the item is ready for return shipping (if a warranty request), or simply issue your refund with the appropriate deductions (if any) once processed. Processing is done first come, first serve, and can take between 1-4 weeks to process (depending on how many requests we currently have in queue). We thank you for your patience while we work on your request.
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